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1 Mixtures and Solutions Candy Chromotography Background Information and Research Biochemists often social occasion this method of separation when studying amino acids or otherwise chemicals from life things. Also they use it to separate a resolve with multiple comp superstarnts with contrasting polarites. People use chromoography to find the things in milk,find out what chemicals atomic number 18 in black ink, also determine whether something is hazardous or not. Purpose The adjudicate of this experiment is to chance what kind of colors and emblazons are in the dulcifys likenessed coating. Also to see what kind of things are in it.Materials Candy with a colo flushed coating, like Skittles or M&Ms (4 different colors) Rubbing alcohol or isopropyl alcohol coffee bean drops (2) Tall glasses or plastic cups (2) Pencil Ruler tape Foil or wallpaper plate Table coarseness water system Toothpicks or cotton wipe ups (4) Measuring cups/spoons Clean pitcher or 2-l iter bottle Procedure 1. Coffee filters usually are round, but its easier to compare your results if the paper is square. So your first task is to cut the coffee filter into a square measuring three cm by nine cm square from a coffee filter.You allow need ii of these l each(prenominal)s to have it away the research laboratory. 2. Space four drops of water (or however many colors you are testing) equally distant on a piece of foil or plate. Position one candy of each color on the drops. confine the color about a minute to come off into the water. expel of the candy. 3. Using a pencil (do not use a pen), campaign a line one cm (1/2 inch) from the edge of one end of one strip of paper. 4. sterilise four pencil dots (one for each color of candy you will be examining) along this line, about 0. cm (1/4 inch) apart. Underneath each dot, label the color of the candy you will test on that spot. You wont have space to write the whole color name, so try abbreviations like B for blue, G for green, etc. 5. out front you complete the rest of the lab, make a prediction of the components you expect to see from each candy color. 6. Dip a toothpick or cotton swab into one sample of colored water and dab the color onto the pencil dot for that color. Use a impertinent toothpick for each color. Try to happen each dot as small as possible.Allow the filter paper to dry, and then go back and add more color to each dot, repeating this step a total of three multiplication for each color. 7. Prepare the salt dissolvent by mixing 1/8 teaspoon of salt and three cups of water in a clean pitcher or 2-liter bottle. Stir or shake the solution until it is dissolved. This will produce a 1% salt solution. 8. Pour the salt solution into a clean tall glass so that the smooth level is 1/4 inch (0. 5 cm). 9. Tape the strip to a pencil and rest the pencil on top of the sway so that the strip hangs into the jar.The goal is to have the end of the chromatography strip only when touching the surface of the solvent solution, with the colored dots above the surface of the liquid. Make sure that the colored spots do not come in direct contact with the liquid in the bottom of the glass. 10. Capillary proceeding will draw the salt solution up the paper. As it passes finished the dots, it will begin to separate the dyes. When the salt water is 1/4 inch (0. 5 cm) from the top edge of the paper, remove it from the glass and pasture it on a clean, flat surface to dry. 11.Repeat steps two through nine (using the second strip of paper and a clean glass) to test the same four colors using the alcohol rather of the saltwater solution at the bottom of the glass. Data and Observations Original Predictions The red M&M will travel the farthest and be the strongest. The The dye in the yellow M&M will travel slight and be the lightest color. pic Other observations I noticed was the stationary phase matters. some(prenominal) the red and yellow travveld to the front but had li ttle separation. Red showed the last-place travelling distance.Discussion and Conclusion The first part of this section discusses the observations and results of the lab as well as any mistakes that may have been do (or what measures were taken to try to avoid mistakes) and what improvements, if any, you can think of for the procedure for the close time the lab will be conducted. The discussion section of this lab should also include discussions on the following A comparison of how each solution (salt water and alcohol) uninvolved the keys from each color of candy or ink type.Which solution worked better at separating each of the pigments (it may be different for different candy colors or inks), and why do you think that is? If you had allowed less time for the lab (stopping the separation process when the solution was half way up the paper) how do you think that would have bear on your results? What pigment colors traveled farther up the paper? What pigment traveled the lea st? Was it the same or different when those pigments appeared in other candy colors or inks? Was it the same or different when separated by alcohol instead of salt water?